Table 1.
Proportion of expressed sequence tags (ESTs) retrieved from arctic-enriched and temperate-enriched Oxytropis subtractive cDNA libraries according to general categories
| Gene category | EST ratio in arctic-enriched librarya (potential false positive)b | EST ratio in temperate-enriched librarya (potential false positive)b |
|---|---|---|
| No similarity | 5.85 × 10−1 (5.91 × 10−3) | 5.84 × 10−2 (0) |
| Unclassified | 3.55 × 10−3 (0) | 2.12 × 10−1 (1.82 × 10−3) |
| ROS related | 5.91 × 10−3 (3.54 × 10−3) | 2.74 × 10−2 (5.47 × 10−3) |
| Histones | 1.18 × 10−3 (1.18 × 10−3) | 2.92 × 10−2 (1.82 × 10−3) |
| DNA-proteins | 0 | 2.00 × 10−2 (0) |
| RNA-proteins (ribosomes) | 2.01 × 10−2 (9.46 × 10−3) | 7.66 × 10−2 (3.65 × 10−3) |
| Response to stimulus | 2.31 × 10−1 (1.19 × 10−1) | 1.08 × 10−1 (1.28 × 10−2) |
| Secondary metabolism | 0 | 1.46 × 10−2 (0) |
| Hormones | 4.73 × 10−3 (4.73 × 10−3) | 3.83 × 10−2 (7.30 × 10−3) |
| Transport | 2.36 × 10−4 (1.18 × 10−3) | 4.01 × 10−2 (7.3 × 10−3) |
| Nucleotides | 0 | 1.82 × 10−3 (0) |
| Signaling | 1.18 × 10−3 (1.18 × 10−3) | 1.82 × 10−2 (1.82 × 10−3) |
| Protein modifications | 1.18 × 10−3 (0) | 2.00 × 10−2 (0) |
| Nitrogen | 1.18 × 10−3 (1.18 × 10−3) | 2.00 × 10−2 (5.47 × 10−3) |
| Lipid | 7.57 × 10−2 (7.47 × 10−2) | 1.46 × 10−2 (3.65 × 10−3) |
| Carbohydrates | 1.18 × 10−3 (0) | 3.65 × 10−2 (0) |
| Energy | 3.55 × 10−2 (1.18 × 10−4) | 2.65 × 10−1 (1.82 × 10−3) |
ROS reactive oxygen species
aThe ratio of ESTs in a category is expressed relative to the total number of ESTs in the subtracted library of origin, as EST ratio = number of EST sequences in library in this category/total number of EST sequences in library
bValues in parenthesis report the potential false positive, identified using similarity of unique genes from one subtracted library to the unique genes of the other subtracted library