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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Bioorg Med Chem Lett. 2011 May 6;21(17):5006–5010. doi: 10.1016/j.bmcl.2011.04.128

Figure 2. HPTLC analysis of gangliosides produced by BJAB and CHO cells cultured with protected ManNAc analogs.

Figure 2

BJAB K20 and CHO Lec3 cells were cultured with protected ManNAc analogs. BJAB K88 and wt CHO cell lines containing UDP-GlcNAc 2-epimerase activity were used as positive controls. Gangliosides were extracted, resolved by HPTLC, and visualized by resorcinol staining. Mobilities of standard gangliosides are indicated on either side of the HPTLC images. Gangliosides appear as doublets, reflecting two different fatty acid lengths that are incorporated into LacCer in these cell lines. (a) BJAB cells generate GM3 and small quantities of GM1a gangliosides. (b) CHO cells produce only GM3 gangliosides. Gangliosides incorporating modified sialic acids have altered mobilities. Incorporation of the modified sialic acids was confirmed by mass spectrometry (supplementary data).