Fig. 1.

C85 cells populations in the course of establishment of methotrexate-induced senescence. Cells were colorimetrically assayed for senescence-associated β-galactosidase activity performed as previously described [8] and stained with Accustain May–Grunwald solution (Sigma-Aldrich). The images were taken with Olympus IX70 microscope at ×100 magnification