Table 1.
Predominant signaling pathways operating in C85 cells during establishment of methotrexate-induced senescence, as inferred from IPA software
| Signaling pathway | Senescence phase | |
|---|---|---|
| Initiation | Maintenance | |
| p53 signaling | 3.36 × 10−8 | 1.72 × 10−2 |
| Interferon signaling | 1.54 × 10−6 | 6.99 × 10−7 |
| Integrin signaling | 8.72 × 10−5 | 8.58 × 10−9 |
| VDR/RXR activation | 1.57 × 10−4 | 1.98 × 10−3 |
| Germ cell–Sertoli cell junction signaling | 1.82 × 10−4 | 1.41 × 10−8 |
| Regulation of actin-based motility by Rho | 1.96 × 10−4 | 6.00 × 10−6 |
| Apoptosis signaling | 2.31 × 10−4 | 3.24 × 10−3 |
| Caveolar-mediated endocytosis | 1.64 × 10−3 | 7.04 × 10−6 |
| p38 MAPK signaling | 1.95 × 10−3 | 1.91 × 10−3 |
| Xenobiotic metabolism signaling | 2.62 × 10−3 | 3.16 × 10−2 |
| Death receptor signaling | 5.53 × 10−3 | 9.60 × 10−6 |
| ERK/MAPK signaling | 9.87 × 10−3 | 5.93 × 10−6 |
| Semaphorin signaling in neurons | 1.00 × 10−2 | 3.78 × 10−6 |
| Production of nitric oxide and reactive oxygen species in macrophages | 1.93 × 10−2 | 9.75 × 10−6 |
| PI3K/AKT signaling | 2.37 × 10−2 | 6.65 × 10−6 |
| Calcium signaling | 5.47 × 10−2 | 3.53 × 10−2 |
| Clathrin-mediated endocytosis | 1.05 × 10−1 | 3.27 × 10−5 |
| ERK5 signaling | 3.38 × 10−1 | 6.85 × 10−3 |
The p value for statistical significance of each pathway is given.