Table 3.

Molecules involved in nitrosative/oxidative stress generation and antioxidant response whose expression is upregulated in C85 cells during establishment of methotrexate-induced senescence

Functional group	Molecules
NO producing enzymes	NOS2A, NOS3
NADPH–oxidase complex regulatory molecule (transmembrane)	NCF4
Phase I xenobiotic detoxification enzymes associated with mitochondrial dysfunction	MAOA, MAOB
Other mitochondrial dysfunction-associated factors	APP, COX6B2, GPD2, KGDH, NDUFAF1, PSEN1
Nrf-2-dependent antioxidant defense enzymes	FTH1, HMOX1, PRDX1, TRXR1, SOD3, SQSTM1

Functional assignment of selected molecules was performed in IPA software. The molecules upregulated during both senescence initiation and maintenance phases are printed bold, upregulated during the initiation phase are printed with a regular font and upregulated during the maintenance phase are underlined APP amyloid β (A4) precursor protein, COX6B2 cytochrome c oxidase subunit VIb polypeptide 2, FTH1 ferritin heavy polypeptide 1, GPD2 glycerol-3-phosphate dehydrogenase 2, HMOX1 heme oxygenase 1 (HO-1), KGDH oxoglutarate dehydrogenase, MAOA/MAOB monoamine oxidase A/B, NCF4 neutrophil cytosolic factor 4 (p40 phox), NDUFAF1 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1, NOS2A/NOS3 inducible (iNOS)/endothelial (eNOS) nitric oxide synthase, PRDX1 peroxiredoxin 1, PSEN1 presenilin 1, TRXR1 thioredoxin reductase 1, SOD3 superoxide dismutase 3, SQSTM1 sequestosome 1. Corresponding microarray data are presented in Table S3