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. 2011 May 15;2(2):369–381. doi: 10.1007/s13148-011-0035-5

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© The Author(s) 2011

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Fig. 3 — The apoptosis phenotype evasion is associated with the loss of Bax activation. a Effect of the folate or PDGFri treatment on the expression levels of BH3-only proteins. Actin is used as loading control. b Relative Bim^mRNA and PUMA^mRNA expressions were estimated by qPCR. Transcriptional FoxO3a activity was estimated by using the pTL-Luc plasmid (Ozyme, France) i.e., a luciferase reporter vector. Western blot (WB) is used to monitor the effect of the folate or PDGFri treatment on the expression levels of FoxO3a and on its phosphorylation. Relative Akt activity was determined by using the PathScan® Phospho-Akt Sandwich ELISA Kit (Ozyme, France). c Effect of the folate, PDGFri, or U0126 treatment on the phosphorylation levels of Bim. Actin is used as loading control. U0126 (0.4 μM, Ozyme, France). Erk1/2 activity was monitored by ELISA method (Ozyme, France)