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Kinetics of the two-step pathway for Gln-tRNAGln formation in the archaeon M. thermautotrophicus. (a) Experimental design used to investigate the possibility that the intermediate Glu-tRNAGln is channeled from GluRSND to GatDE. (b) Summary of rate constants for individual steps in the two-step pathway for Gln-tRNAGln synthesis, derived from experiments presented in thjis manuscript.
