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. Author manuscript; available in PMC: 2012 Sep 2.
Published in final edited form as: J Mol Biol. 2011 Jul 12;411(5):943–950. doi: 10.1016/j.jmb.2011.06.051

Figure 3.

Figure 3

Isoelectric focusing gel of tarantula fibers showing the change in isoelectric focusing point of the myosin RLC between the normal preparation and that obtained using phosphatase inhibitors in the glycerol solutions. Lanes 1 and 2 are from the phosphorylated preparation. Lanes 3 and 4 are from the normal preparation. Both preparations had been stored in rigor/glycerol solution for 2 weeks. The bands are identified by their isoelectric point, which is similar to that of vertebrate skeletal myosin light chains, and by the shift occurring in the phosphorylated preparation. The three bands are identified as unphosphorylated RLC, singly and doubly RLC by comparison with the gels of Hidalgo et. al.8.