Table 1.
Incubationa | Chase | P1b | T1 (sec) | P2 | T2 (sec) | P3 | T3 (sec) | Nc |
---|---|---|---|---|---|---|---|---|
mantATP | ATP + ML-7 | 0.34 ± .02 | 23± 3 | 0.17 ± .02 | 278 ± 31 | 0.48 ± .03 | 1995 ± 231 | 22 |
mantADP | ADP | 0.55 ± .03 | 6 ± 1 | 0.26 ± .02 | 27 ± 3 | 0.11 ± .03 | 193 ± 26 | 11 |
mantATP+ATPd | ATP | 0.79 ± .02 | 9 ± 4 | 0.11 ± .02 | 54 ± 19 | 6 | ||
~Pe mantATP | ATP | 0.48 ± .03 | 15 ± 2 | 0.22 ± .03 | 34 ± 5 | 0.23 ± .02 | 319 ± 29 | 13 |
The first 2 columns give the nucleotides in the initial incubation and the chase solutions.
P1, P2 and P3 are the magnitudes and T1 T2 and T3 are the lifetimes for the first, second and third phases of the three-exponential function that was fit to the data; see Eq. 1, Methods.
Errors are standard errors of the mean for Nc observations.
These data were fit adequately by a two-exponential fit.
~P signifies that the fibers were phosphorylated.