. Author manuscript; available in PMC: 2012 Aug 15.

Published in final edited form as: Chem Res Toxicol. 2011 Jul 20;24(8):1327–1337. doi: 10.1021/tx200218u

Table 1.

Reverse Type I Binding Spectra of P450 1A1, 1A2, and 1B1 Induced by Organoselenium Compounds

P450	chemical	K_s (µM)	ΔA_max (× 10⁻³)	ΔA_max/K_s (× 10⁻³)
P450 1A1	BSC	23 ± 4.1	14 ± 1.0	0.61
	o-XSC	26 ± 4.6	20 ± 1.2	0.77
	m-XSC	30 ± 4.5	18 ± 1.2	0.60
	p-XSC	19 ± 1.6	8.6 ± 0.24	0.45
P450 1A2	BSC	11 ± 2.3	9.5 ± 0.57	0.88
	o-XSC	6.3 ± 1,6	2.4 ± 0.20	0.38
	m-XSC	13 ± 1.9	5.8 ± 0.25	0.46
	p-XSC	12 ± 1.2	9.7 ± 0.40	0.81
P450 1B1	BSC	5.3 ± 0.96	19 ± 0.11	3.60
	o-XSC	4.6 ± 0.75	15 ± 0.51	3.30
	m-XSC	5.7 ± 1.0	21 ± 1.0	3.70
	p-XSC	3.6 ± 0.33	21 ± 0.48	5.83

Spectral interaction was determined in a system containing 1 µM P450 and 6–10 concentrations of selenium compounds in 0.10 M potassium phosphate buffer (pH 7.4) containing 20% glycerol (v/v). Results are presented as means ± SE.