Skip to main content
. Author manuscript; available in PMC: 2012 Aug 15.
Published in final edited form as: Cancer Res. 2011 Jul 5;71(16):5467–5476. doi: 10.1158/0008-5472.CAN-10-3988

Figure 2. Inhibition of PolyI:C induced responses by LPS is mediated by IL-10, Stat3 and partially by P38.

Figure 2

MoDCs were electroporated with STAT3 siRNA and the amount of total protein was measure by western blot (A) and quantified using densitometry (B). These cells were treated with LPS, PolyI:C or their combination, and IL-12p70 was measured (A). MoDCs prior to exposure to LPS, PolyI:C or their combination were pretreated with a P38 inhibitor 10ng/ml or wortmannin 100nM/ml, and IL-12p70 and IL-10 was quantified (B and C respectively). Phosphorylation of P38 was monitored over time upon ligation of TLR4, TLR3 or their combination (D). MoDCs were exposed to 100ng/ml of LPS, 5ug/ml of PolyI:C, 10uM of R848 and/or their combination as well as Mimic cytokine cocktail or left untreated with or without blocking antibodies to IL-10 and IL-10R with or without recombinant IL-10. Cells were then stained with anti CD80 and anti-CD86 (E) anti- PDL-1 antibody (F) or with the isotype control.