MoDCs were monitored for the secretion of IL-12p70 and IL-10 when either untreated or stimulated with LPS, PolyI:C, LPS/PolyI:C, R848, R848/LPS, R848/PolyI:C and Mimic. IL-12p70 and IL-10 was also monitored using the same conditions with or without blocking IL-10 and IL-10R, and with or without pretreatment with recombinant IL-10 (A, B respectively). The same experiment was repeated by monitoring IL-12p70 and IL-10 in supernatants collected over time (C, D respectively). Each experiment was performed in triplicates and repeated at least with 3 different donors.