Table 3.

Plasma and liver concentrations of amino acids and other metabolites in rats fed adequate (n=6), Cys-devoid (n=6) and Met-deficient (n=6) diets for 14 days either without (control) or with (CBHcy) an inhibitor of BHMT activity¹.

		Plasma (µmol/L)		Liver (nmol / mg protein)
Diet	Measure	Control	CBHcy	Control	CBHcy
Adequate	Glutamine	874 ± 29	787 ± 22^*	79 ± 6	58 ± 4^*
	Glycine	182 ± 9	309 ± 5^*	15 ± 1	17 ± 1
	Histidine	33 ± 1	49 ± 2^*	2.8 ± 0.1	3.3 ± 0.1^*
	Methionine	43 ± 2	35 ± 1^*	0.8 ± 0.1	0.7 ± 0.0
	Serine	269 ± 12	240 ± 7	9.3 ± 1.0	4.4 ± 0.2^*
	Ammonia	67 ± 7	67 ± 5	19 ± 1	24 ± 1^*
	Cystathionine	0.7 ± 0.1	2.3 ± 0.2^*	0.2 ± 0.0	0.2 ± 0.0
	Ethanolamine	10 ± 1	8 ± 1	0.9 ± 0.1	0.5 ± 0.0^*
Cysteine-devoid	Glutamine	1087 ± 39	890 ± 42^*	93 ± 6	58 ± 5^*
	Glycine	203 ± 11	344 ± 10^*	21 ± 1	20 ± 1
	Histidine	41 ± 1	55 ± 2^*	3.5 ± 0.2	3.5 ± 0.1
	Methionine	43 ± 1	35 ± 1^*	0.8 ± 0.0	0.6 ± 0.0^*
	Serine	363 ± 14	293 ± 14^*	15 ± 1	6 ± 1^*
	Ammonia	67 ± 4	74 ± 3	20 ± 1	23 ± 1^*
	Cystathionine	0.9 ± 0.1	2.6 ± 0.2^*	0.21 ± 0.02	0.15 ± 0.01^*
	Ethanolamine	8 ± 0	7 ± 1	1.0 ± 0.1	0.5 ± 0.1^*
Methionine-deficient	Glutamine	1134 ± 44	886 ± 48^*	95 ± 6	80 ± 4
	Glycine	196 ± 7	278 ± 19^*	14 ± 1	15 ± 1
	Histidine	42 ± 2	44 ± 2	2.9 ± 0.2	3.5 ± 0.1^*
	Methionine	33 ± 2	20 ± 1^*	0.6 ± 0.0	0.4 ± 0.1^*
	Serine	438 ± 17	309 ± 16^*	13 ± 1	7 ± 0^*
	Ammonia	80 ± 8	69 ± 4	21 ± 0	24 ± 1^*
	Cystathionine	0.5 ± 0.0	0.7 ± 0.1	0.2 ± 0.0	0.2 ± 0.0
	Ethanolamine	8 ± 0	7 ± 0	0.9 ± 0.1	0.4 ± 0.0^*

Values are means ± SEM. Control and CBHcytreatments were compared within sample type using a 1-way ANOVA.

Significant effect of CBHcy treatment over 14 days within sample type (P< 0.05).