Table 4.
Activities of liver Hcy-metabolizing enzymes in ratsfed adequate (n=6), Cys-devoid (n=6) and Met-deficient (n=6) diets for 3 or 14 days either without(control) or with (CBHcy) an inhibitor of BHMT activity1.
| Control |
CBHcy |
||||
|---|---|---|---|---|---|
| Diet | Measure | 3 days | 14 days | 3 days | 14 days |
| BHMT3 | 64.8 ± 4.2 | 72.8 ± 1.8 | 2.4 ± 0.3 | 4.1 ± 0.1 | |
| Adequate | MS2 | 6.4 ± 0.2 | 5.7 ± 0.2 | 6.6 ± 0.2 | 5.1 ± 0.2 |
| CBS2,3,4 | 204.6 ± 19.4 | 220.9 ± 14.6 | 174.7 ± 25.0 | 84.8 ± 4.6 | |
| BHMT3,4 | 46.2 ± 1.6 | 40.2 ± 2.3 | 4.3 ± 0.2 | 6.4 ± 0.3 | |
| Cys-devoid | MS2 | 4.3 ± 0.2 | 3.4 ± 0.0 | 4.7 ± 0.3 | 3.4 ± 0.2 |
| CBS2,3 | 197.6 ± 7.1 | 184.9 ±13.7 | 119.3 ± 6.1 | 70.0 ± 4.5 | |
| BHMT3 | 49.9 ± 3.2 | 51.0 ± 2.5 | 2.1 ± 0.2 | 3.1 ± 0.2 | |
| Met-deficient | MS2,3 | 7.9 ± 0.4 | 6.1 ± 0.2 | 6.3 ± 0.3 | 5.2 ± 0.2 |
| CBS2,3 | 122.3 ±4.6 | 67.6 ± 2.2 | 75.5 ± 5.2 | 17.8 ± 0.9 | |
1
Values are means ± SEM, and all measures are expressed as units of enzyme activity/mg of tissue.
2
Main effect of time (P < 0.05).
3
Main effect of CBHcytreatment (P < 0.05).
4
Time × CBHcytreatment interaction (P < 0.05).