Table 5.
Total liver thiols, SAM and SAHin rats fed adequate (n=6), Cys-devoid (n=6) and Met-deficient (n=6) diets for 3 or 14 days either without (control) or with (CBHcy) an inhibitor of BHMT activity1.
| Control |
CBHcy |
||||
|---|---|---|---|---|---|
| Diet | Measure | 3 days | 14 days | 3 days | 14 days |
| Adequate | Cys | 62.9 ±7 | 57.9 ± 4.0 | 49.0 ± 8.2 | 57.6 ± 1.7 |
| Cys-Gly2 | 14.7 ±1.0 | 20.8 ± 1.5 | 15.8 ± 1.2 | 17.3 ± 0.8 | |
| Glutathione3 | 3981 ± 356 | 4997 ± 145 | 3813 ± 271 | 3581 ± 197 | |
| SAM3 | 51.9 ± 4.5 | 55.2 ± 3.2 | 35.7 ± 1.8 | 29.3 ± 2.9 | |
| SAH3 | 12.0 ± 1.6 | 11.5 ± 1.8 | 10.2 ± 0.5 | 12.1 ± 1.5 | |
| SAM/SAH | 4.5 ± 0.9 | 5.1 ± 1.1 | 3.6 ± 0.2 | 2.5 ± 0.2 | |
| Cys-devoid | Cys2 | 60.2 ±2.9 | 81.3 ±3.8 | 64.2 ± 3.0 | 78.5 ± 1.8 |
| Cys-Gly3 | 14.5 ± 1.2 | 17.3 ± 1 | 12.3 ± 0.9 | 14.6 ± 1.7 | |
| Glutathione2,3 | 1999 ± 270 | 2681 ± 310 | 1740 ± 227 | 2273 ± 287 | |
| SAM3 | 56 ± 2.4 | 59.8 ± 1.8 | 35.1 ± 2.1 | 37.0 ± 1.6 | |
| SAH3 | 9.0 ± 0.9 | 6.3 ± 0.3 | 16.3 ± 2.7 | 18.2 ± 1.9 | |
| SAM/SAH3 | 6.5 ± 0.5 | 9.7 ± 0.7 | 2.3 ± 0.1 | 2.0 ± 0.2 | |
| Met-deficient | Cys | 66.8 ±12.8 | 72.6 ± 4.4 | 64.3 ± 9.9 | 81.0 ± 4.5 |
| Cys-Gly | 18.4 ± 3.6 | 18.6 ± 1.6 | 18.0 ± 2.6 | 21.5 ± 2.5 | |
| Glutathione | 3860 ± 437 | 3797 ± 228 | 4024 ± 271 | 4097 ± 353 | |
| SAM3 | 44.7 ± 1.7 | 49.3 ± 4.3 | 23.2 ± 2.0 | 21.0 ± 2.8 | |
| SAH3 | 6.2 ± 0.5 | 7.5 ± 0.4 | 7.3 ± 0.5 | 8.2 ± 0.9 | |
| SAM/SAH2,3,4 | 7.5 ± 0.8 | 6.6 ± 0.4 | 3.2 ± 0.3 | 2.7 ± 0.3 | |
1
Values are means ± SEM. Total liver thiols, SAM and SAH measures are expressed as nmol/g of liver tissue and the ratio of SAM/SAH is in arbitrary units.
2
Main effect of time (P < 0.05).
3
Main effect of CBHcytreatment (P < 0.05).
4
Time × CBHcytreatment interaction (P < 0.05).