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A) Methyl-glyoxal levels in sciatic nerve tissue. Sciatic nerve tissue was retrieved and subjected to HPLC analysis for detection of levels of pre-AGE methyl-glyoxal (MG). As shown, hyperglycemic animals had significantly higher level of MG compared to the controls, *P < 0.05. B) Plasma AGE levels. AGE levels were measured by ELISA in the plasma and compared with the baseline level to determine the degree of glycation occurring during sustained hyperglycemia.
