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. Author manuscript; available in PMC: 2012 Jul 12.
Published in final edited form as: Curr Biol. 2011 Jun 30;21(13):1158–1165. doi: 10.1016/j.cub.2011.06.015

Figure 1. Efficient mitotic error correction, resistance to Aurora B inhibition, and enrichment of Aurora B at misaligned centromeres in healthy, diploid cells but not in aneuploid cells.

Figure 1

(A) The percentage of HeLa and RPE cells containing misaligned chromosomes was quantified at 45 min following monastrol withdrawal. Error bars represent standard error of the mean.

(B) The percentage of each cell type containing misaligned chromosomes with an intermediate dose of ZM (500 nM) at one hour following monastrol withdrawal. Error bars represent standard error of the mean.

(C–F) Representative images of RPE (C), FF (D), HeLa (E), and U87MG (F) cells stained for Aurora B with misaligned chromosomes and a clearly discernable metaphase plate are shown. Insets show 4X magnified views of the boxed area. Quantitation of Aurora B levels is shown for each cell line at misaligned (blue) and aligned (red) chromosomes. Error bars represent standard error of the mean. Scale bars = 2 µm.

(G) Each cell line is plotted as Aurora B enrichment on the centromeres of misaligned chromosomes versus the chromosome number per cell (each data point represents a single cell).