Skip to main content
. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: J Immunol. 2010 Nov 24;186(1):516–526. doi: 10.4049/jimmunol.1000955

Figure 1. IL-5 and IL-3 prime chemotactic factor-induced phosphorylation of ERK1/2 and Akt.

Figure 1

A. Purified human blood eosinophils were primed with control buffer, IL-5 or IL-3 for 1 hr, followed by stimulation with fMLP or control for the indicated time points. Cell lysates were processed by SDS-PAGE and immunoblotted for pERK1/2 and actin (A, N=3) as detailed under Materials and Methods. B and C. Eosinophils were primed with control buffer or IL-5 for 1 hr followed by a two-min stimulation with fMLP or CCL5. Cell lysates were subjected to SDS-PAGE and immunoblotted for pERK1/2 (B, N=5), pAkt (C, N=4) and Grb2.