Skip to main content
. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: J Immunol. 2010 Nov 24;186(1):516–526. doi: 10.4049/jimmunol.1000955

Figure 7. PILR inhibits fMLP-stimulated Ras activation following IL-5 priming in human blood eosinophils.

Figure 7

Human primary eosinophils were preincubated with 10 μM control peptide or PILR for 30 min. The cells were then primed with 100 pM IL-5 or buffer control for 1 hr followed by 100 nM fMLP for 2 min. Cells lysates were subjected to the RBD pull-down assay as detailed under Materials and Methods. An aliquot of the whole cell lysate (WCL) from each treatment was removed to assess levels of pERK1/2 and actin (upper panel). The levels of active Ras were detected in the pull-down samples via immunobloting (upper panel) and normalized to that of actin in the whole cell lysates. The mean ± (SEM) from four independent experiments are summarized in the lower panel.