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. 2011 May 4;1:20. doi: 10.1186/2044-5040-1-20

Figure 2.

Figure 2

Sequence alignments between uncharacterized human open reading frames (ORF) and Torpedo cDNA. Two human ORFs were identified by tandem mass spectrometry (MS/MS) analysis of electric organ fractions by the SEQUEST algorithm in BioWorks 3.3.1 software, crossreferencing our in-house Torpedo californica cDNA library translated into six reading frames. Comprehensive alignments of nucleotide and protein sequences between uncharacterized human ORFs (blue text) and Torpedo cDNA (black text) were compiled from individual ClustalW alignments (default parameters with gonnet matrix) for C1orf123 (a) and C6orf130 (b). ClustalW protein alignment is shown separately to highlight protein sequence similarity with the translated cDNA sequence (Expasy translate tool) and peptides identified by mass spectral mapping (highlighted in red). Start and stop amino acids are highlighted in yellow.