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. 2011 Aug;13(8):676–684. doi: 10.1593/neo.11512

Figure 5.

Figure 5

SIAH1 regulated by EHMT2 is a key regulator of cancer cell growth and apoptosis. (A) Western blot analyses in SBC5 cells after treatment with siRNAs targeting EGFP (control; siEGFP), EHMT2 (siEHMT2 #2), and SIAH1 (siSIAH1) for 72 hours. Anti-PARP1 (sc-8007; Santa Cruz) and -cleaved caspase 3 (no. 9661S; Cell Signaling, Danvers, MA) antibodies were used as apoptosis markers, and anti-GAPDH antibody was used as an internal control. (B) Cell growth assay of SBC5 cells treated with indicated siRNAs. siEHMT2 #2 and either siEGFP or siSIAH1 were transfected 24 hours after preparation of cells, and subsequently, cell viability was measured 48 and 96 hours after siRNA treatment. Mean ± SD of three independent experiments. P values were calculated using Student's t test (**P < .01). (C) Colony formation assay of SBC5 cells. Indicated siRNAs were transfected 24 hours after preparation of cells, and Giemsa staining was performed 96 hours after treatment with siRNAs.