Table 2. ITC derived thermodynamic profiles for the binding of ADG and DAU to tRNA at different Na+ concentrations in CP buffer at 20°C.
Drug | NaCl (mM) | K/104 M−1 | N | ΔG/kcal mol−1 | ΔH/kcal mol−1 | TΔS/kcal mol−1 | ΔGt/kcal mol−1 | ΔGpe/kcal mol−1 | ZΨ |
10 | 9.9±0.5 | 2.04±0.02 | −6.74±0.05 | −1.35±0.01 | 5.40±0.01 | 5.31±0.01 | 1.43±0.02 | ||
ADG | 20 | 6.5±0.3 | 2.10±0.02 | −6.50±0.04 | −1.02±0.01 | 5.42±0.01 | 5.28±0.02 | 1.21±0.03 | −0.53±0.04 |
50 | 4.2±0.1 | 2.40±0.03 | −6.24±0.02 | −0.58±0.02 | 5.62±0.02 | 5.31±0.02 | 0.93±0.03 | ||
10 | 38.5±0.3 | 1.09±0.02 | −7.54±0.02 | −1.71±0.02 | 5.71±0.01 | 5.03±0.01 | 2.51±0.02 | ||
DAU | 20 | 22.2±0.1 | 1.15±0.05 | −7.21±0.03 | −1.42±0.02 | 5.72±0.02 | 5.08±0.02 | 2.13±0.01 | −0.93±0.01 |
50 | 8.40±0.2 | 1.36±0.02 | −6.64±0.03 | −0.48±0.02 | 6.12±0.03 | 5.01±0.01 | 1.63±0.01 |
The data in this table are derived from ITC experiments conducted in CP buffer. K and ΔH values were determined from fits of the ITC profiles, with indicated errors reflecting standard deviations of the experimental data from the fitted curves. The value of ΔG were determined using the equation ΔG = −(RTlnK). The values of TΔS were determined using equation TΔS = ΔH-ΔG. The uncertainties in the determinations are indicated. The ITC profiles were fit with a model for single binding site.