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. 2011 Aug 16;6(8):e23521. doi: 10.1371/journal.pone.0023521

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Bonomelli et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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MALDI-TOF MS analyses of released desialylated N-linked glycans ([M+Na]⁺ ions) from: (A) recombinant monomeric gp120_JRCSF expressed in HEK 293T cells; (B, C and D) respectively gp160_JRCSF, gp120_JRCSF and soluble, non-virion associated envelope gp120_JRCSF isolated from pseudoviral particle preparations generated by transfection of HEK 293T cells with the pSVIII-JRCSF and pSG3Δenv plasmids at a ratio of 1∶10; (E) gp120_JRCSF isolated from replication competent viral particles generated by transfection of HEK 293T cells with pLAI-JRCSF env molecular clone; (F, G and H) respectively gp120_92RW009, gp120_JRCSF and gp120_93IN905 isolated from virus obtained by infection of human PBMCs. Symbols used for the structural formulae in this and subsequent figures: ⋄ = Gal, ▪ = GlcNAc, ○ = Man, = Fuc [46]. The linkage position is shown by the angle of the lines linking the sugar residues (vertical line = 2-link, forward slash = 3-link, horizontal line = 4-link, back slash = 6-link). Anomericity is indicated by full lines for β-bonds and broken lines for α-bonds [46]. The oligomannose series are highlighted.

Inline graphic — MALDI-TOF MS analyses of released desialylated N-linked glycans ([M+Na]⁺ ions) from: (A) recombinant monomeric gp120_JRCSF expressed in HEK 293T cells; (B, C and D) respectively gp160_JRCSF, gp120_JRCSF and soluble, non-virion associated envelope gp120_JRCSF isolated from pseudoviral particle preparations generated by transfection of HEK 293T cells with the pSVIII-JRCSF and pSG3Δenv plasmids at a ratio of 1∶10; (E) gp120_JRCSF isolated from replication competent viral particles generated by transfection of HEK 293T cells with pLAI-JRCSF env molecular clone; (F, G and H) respectively gp120_92RW009, gp120_JRCSF and gp120_93IN905 isolated from virus obtained by infection of human PBMCs. Symbols used for the structural formulae in this and subsequent figures: ⋄ = Gal, ▪ = GlcNAc, ○ = Man, = Fuc [46]. The linkage position is shown by the angle of the lines linking the sugar residues (vertical line = 2-link, forward slash = 3-link, horizontal line = 4-link, back slash = 6-link). Anomericity is indicated by full lines for β-bonds and broken lines for α-bonds [46]. The oligomannose series are highlighted.