Table 1. Abundances of released N-linked glycans obtained from recombinant (monomeric), pseudoviral, and viral gp120†.
gp120 source | Cell-type | Man5-9GlcNAc2% | Man5GlcNAc2% | Complex% | Mannose content relative to rgp120‡ |
Recombinant monomer (pHLsec JRCSF) | 293T | 29% | 7.7% | 71% | 1.0 |
Pseudovirus (pSG3Δenv:pSVIII JRCSF, 2∶1) | 293T | 98% | 38% | 2% | 3.4 |
Pseudovirus (pSG3Δenv:pSVIII JRCSF, 10∶1) | 293T | 85% | 39% | 15% | 2.9 |
Supernatant (pSG3Δenv:pSVIII JRCSF, 10∶1) | 293T | 73% | 18% | 27% | 2.5 |
Virus (pLAI-JRCSF env) | 293T | 56% | 10% | 44% | 1.9 |
Virus JRCSF (clade B) | PBMC | 79% | 12% | 21% | 2.7 |
Virus 92RW009 (clade A) | PBMC | 64% | 10% | 36% | 2.2 |
Virus 93IN905 (clade C) | PBMC | 62% | 19% | 38% | 2.1 |
†Abundances obtained for desialylated N-linked glycans released from gp120 described in this study. Values were obtained from data presented in Figure 1 and Doores et al. [11].
‡Values represent the increase in oligomannose population (Man5-9GlNAc2) for pseudoviral and viral gp120 compared to monomeric, recombinant gp120.