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. 2011 Aug 16;6(8):e23521. doi: 10.1371/journal.pone.0023521

Table 1. Abundances of released N-linked glycans obtained from recombinant (monomeric), pseudoviral, and viral gp120.

gp120 source Cell-type Man5-9GlcNAc2% Man5GlcNAc2% Complex% Mannose content relative to rgp120
Recombinant monomer (pHLsec JRCSF) 293T 29% 7.7% 71% 1.0
Pseudovirus (pSG3Δenv:pSVIII JRCSF, 2∶1) 293T 98% 38% 2% 3.4
Pseudovirus (pSG3Δenv:pSVIII JRCSF, 10∶1) 293T 85% 39% 15% 2.9
Supernatant (pSG3Δenv:pSVIII JRCSF, 10∶1) 293T 73% 18% 27% 2.5
Virus (pLAI-JRCSF env) 293T 56% 10% 44% 1.9
Virus JRCSF (clade B) PBMC 79% 12% 21% 2.7
Virus 92RW009 (clade A) PBMC 64% 10% 36% 2.2
Virus 93IN905 (clade C) PBMC 62% 19% 38% 2.1

†Abundances obtained for desialylated N-linked glycans released from gp120 described in this study. Values were obtained from data presented in Figure 1 and Doores et al. [11].

‡Values represent the increase in oligomannose population (Man5-9GlNAc2) for pseudoviral and viral gp120 compared to monomeric, recombinant gp120.