Table 1. PCR and sequencing primers for CYP1B1 gene analysis.
Primer name | Primer sequence (5'→3') | Size (bp) |
---|---|---|
CYP1B1 1F | TCTCCAGAGAGTCAGCTCCG | 786 |
CYP1B1 1R | GGGTCGTCGTGGCTGTAG | |
CYP1B1 2F | ATGGCTTTCGGCCACTACT | 787 |
CYP1B1 2R | GATCTTGGTTTTGAGGGGTG | |
CYP1B1 3F | AGTGAGAAATTAGGAAGCTGTTTTAGA | 594 |
CYP1B1 3R | GCCAGGATGGAGATGAAGAG | |
CYP1B1 4F | CCCAAGGACACTGTGGTTTT | 498 |
CYP1B1 4R | AACGCTAATTGAGAAGCAGCA |
Annealing temperature, 60 °C for all primer pairs.