Table 1. Primers used for amplification and sequencing of PAX6.
| Primer ID | Sequence (5′-3′) | Product length (bp) | Annealing Temperature (°C) |
|---|---|---|---|
| Extra-E1-F* |
GAGCTGTGCCCAACTCTAGC |
|
|
| Extra E1-R |
TCCATCTTTGTATGCCTCCTTT |
399 |
56 |
| Exon1F |
GGAGAGGGAGCATCCAATC |
|
|
| Exon1R |
TCCTGGGAAGGAGACAGAGA |
396 |
56 |
| Exon2F |
ACACACTTGAGCCATCACCA |
|
|
| Exon2R |
CTCCTGCGTGGAAACTTCTC |
467 |
56 |
| Exon3F |
AGAGAGCCCATGGACGTATG |
|
|
| exon3R |
CCCAATCTGTTTCCCCTACA |
318 |
56 |
| Exon4F |
TGCAGCTGCCCGAGGATTA |
|
|
| Exon4R |
GCACCCCGAGCCCGAAGTC |
144 |
66 |
| Exon5F |
TCCCTCTTCTTCCTCTTCACT |
|
|
| Exon5R |
GGGGTCCATAATTAGCATC |
301 |
61 |
| Exon6–7F# |
GCTCTCTACAGTAAGTTCTC |
|
|
| Exon6–7R |
AGGAGAGAGCATTGGGCTTA |
457 |
61 |
| Exon8F |
GATTTGCAGGTGTCATCAAT |
|
|
| Exon8R |
ATATGGAGAGCTGCGTGGAT |
212 |
65 |
| Exon9F |
TTTGGTGAGGCTGTCGGGA |
|
|
| Exon9R |
TCTTTGTACTGAAGATGTGGC |
339 |
58 |
| Exon10F |
GTAGTTCTGGCACAATATGG |
|
|
| Exon10R |
GTACTCTGTACAAGCACCTC |
206 |
62 |
| Exon11–12F |
GGCTCGACGTAGACACAGT |
|
|
| Exon11–12R |
TGCAGACACAGCCAATGAGG |
500 |
62 |
| Exon13F |
GCTGTGTGATGTGTTCCTCA |
|
|
| Exon13R |
AAGAGAGATCGCCTCTGTG |
245 |
62 |
| Exon14F |
CATGTCTGTTTCTCAAAGGG |
|
|
| Exon14R | CCATAGTCACTGACTGAATTAACAC | 202 | 61 |
#Exon numbers are based on the current version of gene structure and NM_001604.4, where the original exon 5a is numbered as exon 6. *This extra exon is based on another transcript variant NM_001127612.1, which is not present in the trancript variant of NM_001604.4.