Table 1. Sequences of the primers used for reverse transcription polymerase chain reaction.
| Primers | Sequence (5´→3´) | Product size (bp) | Annealing temperature (ºC) | PCR cycles |
|---|---|---|---|---|
|
ADAMTS-1 |
(F) CAGTACCAGACCTTGTGCAGACCTT |
299 |
58 |
37 |
| |
(R) CACACCTCACTGCTTACTGGTTTGA |
|
|
|
|
SDF-1α |
(F) TGCCCCTGCCGGTTCTTCGAG |
311 |
58 |
37 |
| |
(R) CTGTTGTTGTTCTTCAGCCGTGCAA |
|
|
|
|
VEGF |
(F) CTGCTGTACCTCCACCATGCCAAGT |
509 |
57 |
37 |
| |
(R) CTGCAAGTACGTTCGTTTAACTCA |
|
|
|
|
TGF-β |
(F) CGGGGCGACCTGGGCACCATCCATGAC |
405 |
57 |
37 |
| |
(R) CTGCTCCACCTTGGGCTTGCGACCCAC |
|
|
|
|
MMP-2 |
(F) GAGTTGGCAGTGCAATACCT |
666 |
57 |
38 |
| |
(R) GCCATCCTTCTCAAAGTTGT |
|
|
|
|
MMP-9 |
(F) AGTTTGGTGTCGCGGAGCAC |
754 |
57 |
37 |
| |
(R) TACATGAGCGCTTCCGGCAC |
|
|
|
|
TNF-α |
(F) CAGCCTCTTCTCATTCCTGCTTGTG |
511 |
58 |
36 |
| |
(R) CTGGAAGACTCCTCCCAGGTATAT |
|
|
|
|
TSP-1 |
(F) ACCAAAGCCTGCAAGAAAGA |
311 |
57 |
37 |
| |
(R) ATGCCATTTCCACTGTAGCC |
|
|
|
|
TSP-2 |
(F) CAGAGTACTGGCGTCGGTCA |
649 |
57 |
37 |
| |
(R) ATAAGATCGCAGCCCACATACAG |
|
|
|
|
CXCR4 |
(F) ATGTAGACACTGGCGGAAATGG |
459 |
57 |
37 |
| |
(R) AGGTGGGGCGAAAGGAAAC |
|
|
|
|
Actb |
(F) TGTGATGGTGGGAATGGGTCAG |
514 |
55 |
25 |
| (R) TTTGATGTCACGCACGATTTCC |
All primers used were purchased from Genset Oligos (Kyoto, Japan). The amplification was performed using a GeneAmp® PCR System 9700 (Perkin-Elmer, Foster City, CA). In the table, "F" indicates forward primer and "R" indicates reverse primer.