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. 2011 Aug 16;6(8):e23447. doi: 10.1371/journal.pone.0023447

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Chaves et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Quantitative RT-PCR analysis of photolyase mRNA levels in the laser-microdissected SCN of PtCPD-PL and At(6-4)PP-PL transgenic mice. Left panel: Ethidium bromide stained gel of PCR amplified cDNA, obtained from two independent transgenic mice and corresponding wild type littermates (sacrificed at ZT3). Right panel: graphic representation of quantitative RT-PCR amplification data from two independent animals per genotype (see Experimental procedures for details). The Y-axis represents the PtCPD-PL and At(6-4)PP-PL mRNA levels relative to that of Hprt. (B, C) Circadian behavior of At(6-4)PP-PL (B) and PtCPD-PL (C) transgenic mice and corresponding littermates (n = 10 per genotype). Animals were kept under normal light conditions (LD 12∶12 h) and subsequently exposed to constant darkness (DD) (indicated on the right side of the panels). Shown are representative examples of double-plotted actograms and graphic representations of the free-running period (τ) in constant darkness (bottom panels). Error bars represent the standard error of the mean (SEM); the asterisk indicates significance (p = 0.03).