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. 2011 Aug 16;6(8):e23447. doi: 10.1371/journal.pone.0023447

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Chaves et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Representative examples of bioluminescence rhythms in liver slices obtained from mice hydroporated with pGL4.11-Bmal1::Luc (black line), together with either empty pcDNA3 (blue line) or pcDNA-CPD photolyase constructs (green line). (A, B )Liver slices from control mice, injected with the reporter construct only. (C-H) Liver slices from Cry1^-/-/Cry2^-/- mice injected with either empty pcDNA3 (C, D, H) or pcCry1::PtCPD-PL (E-G), in addition to the reporter construct. In some experiments (G and H) the slices were treated with Forskolin at 96 h to resynchronize the circadian clock. The Y-axis represents base line subtracted bioluminescence values.