Figure 4.

Impact of ABCC4 suppression in SH-SY5Y neuroblastoma cells. A) Western blot analysis of ABCC4 protein expression following exposure of SH-SY5Y cells to ABCC4-specific SMARTpool siRNA, or to control siRNA. B) Enhanced neurite extension in SH-SY5Y cells upon depletion of ABCC4. C) ³H-thymidine incorporation in SH-SY5Y cells depleted of ABCC4. D) Clonogenic capacity upon depletion of ABCC4 was assayed after 12 days. In panel (B) and (D), P values were derived from two-sided Student t test vs control, whereas one-sample t test was used in panel (C) (H₀, μ = 100%). Means are derived from three replicate experiments and error bars represent 95% confidence intervals. ABCC4 = ATP-binding cassette, subfamily C, member 4; siRNA = short interfering RNA.