Skip to main content
. Author manuscript; available in PMC: 2012 Aug 24.
Published in final edited form as: J Proteomics. 2011 Apr 13;74(9):1701–1710. doi: 10.1016/j.jprot.2011.03.035

Fig 4.

Fig 4

Purification of rPV24 using Ni-NTA chromatography (a) SDS-PAGE analysis and (b) chemiluminescence analysis of immunoblot using anti-His antibody. Lane 1 = molecular weight standards, lane 2 = whole lysate, lane 3 = soluble fraction, lane 4 = flow-through fraction, lane 5 = last washing fraction, and lane 6 and 7 = eluted fractions (c) Immunoblot analysis of rPV24 with the immune serum from a P vivax patient. Lane 1 = molecular weight standards, lane 2 = Ponceu S staining of rPV24 protein, and lane 3 = immunoblot of rPV24 Arrows indicate purified rPV24.