Table 2.
Diffusion coefficients of the AS1- and AS2-containing peptides employed in this study.
Diffusion coefficient (10-10 m2/s)a | |||||||
---|---|---|---|---|---|---|---|
Buffer |
DMPC/DHPC |
[DMPC/DMPG 4:1]/DHPC |
|||||
Peptide | Dfree | Dcomplex | Dbicelle | x (%)b | Dcomplex | Dbicelle | x (%)b |
AS1p-Af1503 | 1.6 ± 0.07 | 1.2 ± 0.07 | 0.38 ± 0.01 | 36 | 0.96 ± 0.1 | 0.36 ± 0.01 | 52 |
AS2p-Af1503 | 1.6 ± 0.02 | 0.39 ± 0.01 | 0.37 ± 0.01 | 98 | 0.40 ± 0.01 | 0.37 ± 0.01 | 98 |
AS1p-EnvZEc | 1.6 ± 0.05 | 1.2 ± 0.04 | 0.38± 0.02 | 36 | 1.1 ± 0.04 | 0.39 ± 0.01 | 43 |
AS2p-EnvZEc | 1.6 ± 0.04 | 1.0 ± 0.05 | 0.35 ± 0.01 | 48 | 1.1 ± 0.03 | 0.37 ± 0.01 | 40 |
AS1p-NarXEc | 1.5 ± 0.05 | 0.40 ± 0.01 | 0.38 ± 0.01 | 98 | 0.35 ± 0.01 | 0.36 ± 0.01 | 100 |
AS2p-NarXEc | 1.6 ± 0.04 | 1.0 ± 0.02 | 0.37 ± 0.01 | 49 | 0.92 ± 0.02 | 0.37 ± 0.01 | 58 |
AS1p-TarEc | 1.4 ± 0.04 | 0.38 ± 0.01 | 0.37 ± 0.01 | 99 | 0.35 ± 0.01 | 0.36 ± 0.01 | 100 |
AS2p-TarEc | 1.7 ± 0.06 | 1.2 ± 0.04 | 0.38 ± 0.01 | 42 | 1.2 ± 0.05 | 0.37 ± 0.01 | 40 |
Diffusion coefficients in D2O normalized according to the diffusion of HDO in order to account for differences of viscosity.
Estimation of the percentage of peptide bound to the phospholipid bicelle as calculated by equation 1. Significantly different values are indicated in bold font.