Table 1.

The 95C5 complementation construct was created by ligating five different PCR products into the pCR2.1-TOPO vector (Invitrogen). The name of the primer indicates which piece it amplifies, the direction of the primer as it relates to the gene, and the restriction endonuclease used for cloning. For instance, the primer P1-F-KpnI is the forward primer used to create the first PCR fragment and was cloned using KpnI. Pieces 1, 2, and 5 were amplified using genomic DNA and pieces 3 and 4 were amplified from cDNA.

Primer name	Primer sequence
P1-F-KpnI	5’-GAAGGTACCTTCGTCTGTCCACAAT-3’
P1-R-ClaI	5’-GCAGAGGCGCCGAGACAAAGACG-3’
P2-F-ClaI	5’-GTAGTCAACCGCCCCACATCAG-3’
P2-R-NotI	5’-CCGGTATGCTGGGCTGTATGTA-3’
P3-F-NotI	5’-GCAGACGAGCAAGGACCAGGAT-3’
P3-R-HindIII	5’-CGGCACGCGTCGATACACCT-3’
P4-F-HindIII	5’-GCAGAGGCGAGCGACAACT-3’
P4-R-PacI	5’-TTAATTAAGCCATCAGCCCCGTCAC-3’
P5-F-PacI	5’-GGCTTAATTAATGGAGAAAGGGTGTC-3’
P5-R-XbaI	5’-GGGTCGACGCCGGAACATAAG-3’