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Activation of apo-nNOS with purified activating reagents. Purified apo-nNOS was incubated for 1 h at 30 °C with the additions noted under each column and nNOS activity was assayed. YM-1 (25 μM), MKT-077 (25 μM), thioredoxin (Trx, 25 μM), and BMC (3 mM) were present at their maximally effective concentrations.
