Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Activation of purified apo-nNOS by Hsp70 and thioredoxin. During apo-nNOS purification a disulfide is formed within the heme/substrate binding cleft favoring a closed state of the cleft. Hsp70 binding traps an open state of the cleft to facilitate thioredoxin mediated generation of thiols, with subsequent heme entry and ligation.
