Table 4.
Effects of O2 in dCTP
| dNTP | DNA-N | Vmax | Km | Vmax/Km | Discriminationa |
|---|---|---|---|---|---|
| (primer elongation min−1) (μM) (elongation μM−1 min−1) | |||||
| a)Pol α | |||||
| d6AmPy | DNA-A | 0.83 (0.03) | 99 (9) | 0.0084 | 220 |
| d6AmPy | DNA-T | L | L | 0.037 | 270 |
| d6AmPy | DNA-C | L | L | 0.0096 | 1100 |
| d6AmPy | DNA-G | nd | nd | nd | >10000 |
| d6MPy | DNA-A | 0.31 (0.05) | 675 (210) | 0.0005 | 4000 |
| d6MPy | DNA-T | nd | nd | nd | >10000 |
| d6MPy | DNA-C | nd | nd | nd | >10000 |
| d6MPy | DNA-G | nd | nd | nd | >10000 |
| d6ClPy | DNA-A | 1.03 (0.03) | 43 (3) | 0.024 | 76 |
| d6ClPy | DNA-T | nd | nd | nd | >10000 |
| d6ClPy | DNA-C | nd | nd | nd | >10000 |
| d6ClPy | DNA-G | nd | nd | nd | >10000 |
| dC | DNA-A | 0.22 (0.01) | 432 (65) | 0.0005 | 3700 |
| dC | DNA-T | 0.18 (0.01) | 67 (8) | 0.0027 | 4100 |
| dC | DNA-C | nd | nd | nd | >10000 |
| b)Herpes pol | |||||
| 6AmPy | DNA-A | 0.075 (0.007) | 120 (40) | 0.00063 | 2100 |
| 6AmPy | DNA-T | nd | nd | nd | >20000 |
| 6AmPy | DNA-C | nd | nd | nd | >20000 |
| 6AmPy | DNA-G | 0.043 (0.04) | 110 (40) | 0.0004 | 2800 |
| 6ClPy | DNA-A | 0.71 (0.04) | 1900 (200) | 0.00037 | 3700 |
| 6ClPy | DNA-T | nd | nd | nd | >20000 |
| 6ClPy | DNA-C | nd | nd | nd | >20000 |
| 6ClPy | DNA-G | nd | nd | nd | >20000 |
| 6MePy | DNA-A | L | L | 0.00011 | 12000 |
| 6MePy | DNA-T | nd | nd | nd | >20000 |
| 6MePy | DNA-C | nd | nd | nd | >20000 |
| 6MePy | DNA-G | nd | nd | nd | >20000 |
a
Discrimination values reflect the efficiency of dNTP incorporation compared to the incorporation efficiency of the corresponding natural base pair. nd: not detectable. L: the rate increased linearly with increasing dNTP concentration, thus making it impossible to calculate Km or Vmax. Vmax/Km was calculated from the slope of the line. Values in parentheses are the standard deviations.