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. Author manuscript; available in PMC: 2012 Aug 23.
Published in final edited form as: Biochemistry. 2011 Jul 26;50(33):7243–7250. doi: 10.1021/bi2006916

Table 5.

Effects of O2 in the template

dNTP DNA-N Vmax Km Vmax/Km Discriminationa
(primer elongation min−1) (μM) (elongation μM−1 min−1)
Pol α
dA DNA-6OPy 0.38 (0.03) 4 (1) 0.093 120
dT DNA-6OPy 0.11 (0.01) 66 (16) 0.0016 6900
dG DNA-6OPy 0.10 (0.01) 71 (16) 0.0014 7700
dC DNA-6OPy nd nd nd >10000
dA DNA-6AmPy 0.8 (0.1) 642 (184) 0.0012 8100
dT DNA-6AmPy 0.12 (0.01) 73 (20) 0.0016 6400
dC DNA-6AmPy nd nd nd >10000
dG DNA-6AmPy 0.12 (0.01) 0.16 (0.33) 0.74 14
a

Discrimination values reflect the efficiency of dNTP incorporation compared to the incorporation efficiency of the corresponding natural base pair. nd: not detectable. Values in parentheses are the standard deviations.