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. Author manuscript; available in PMC: 2012 Aug 23.
Published in final edited form as: Biochemistry. 2011 Jul 26;50(33):7243–7250. doi: 10.1021/bi2006916

Table 6.

Effects of removing N-3 from a templating dA.

dNTP DNA-N Vmax Km Vmax/Km Discriminationa
(primer elongation min−1) (μM) (elongation μM−1 min−1)
Pol α
dA DNA-3DA 0.13 (0.02) 134 (66) 0.0010 1900
dT DNA-3DA 33 (2) 178 (30) 0.037 50
dC DNA-3DA nd nd nd >10000
dG DNA-3DA nd nd nd >10000
a

Discrimination values reflect the efficiency of dNTP incorporation compared to the incorporation efficiency of the corresponding natural base pair. nd: not detectable. Values in parentheses are the standard deviations.