Figure 1. Generation of K562-based aAPC that expresses DP4 as a single HLA allele.
The surface expression of HLA-DR, DP, CD80, and CD83 on DP4-aAPC and parental K562 was analyzed by flow cytometry following staining with specific mAbs. Note that K562 endogenously expresses a modest level of CD80 on the cell surface.