Figure 6. Long-term in vitro culture using DP4-aAPC does not induce the expression of Foxp3.
DP4-restricted Survivin-specific CD4+ T cells (Patients B2, C1 and D) were analyzed for the intracellular expression of Foxp3. Total CD4+ T cells in culture were gated. Purified CD4+ T cells from the same donor prior to stimulation were also studied for comparison.