Figure 4.

Augmented NO production in BAE-1 cells by WT-CST and P370L-CST. (A) Normalized fluorescence of DAR-4M AM (left traces) and Fluo3 AM (right traces) on single BAE-1 cells. WT-CST enhanced NO production without affecting intracellular calcium concentration. (B) In BAE-1 cells pretreated with Wm, WT-CST failed to induce NO synthesis stimulation. Bars in the time courses indicate the times of drugs application. (C) Bar graphs representing the percent variation respect to control of the first derivative values of the DAR-4M AM signal (to evidentiate changes in NO synthesis, left panel) and the percent variation respect to control of the normalized Fluo3 AM signal (right panel), in the different experimental conditions.