Figure 1.

A, Increased permeabilization (PI staining) with 0.1 μg/mL PLY in Ca-free buffer. B, Increased LDH release by equivalent PLY amounts in Ca-rich and Ca-free buffer. C, Depletion of Mg from the Ca-free buffer does not alter the toxicity of 0.1 μg/mL PLY, implying that the effects are Ca specific. D, Reduction of extracellular Ca from 2 to 1 mM enhanced the lytic capacity of 0.1 μg/mL PLY. ** P < .01, Mann–Whitney U test (see Methods). All values in the figures represent mean ± SEM, n = 5; PI, propidium iodide; Mg, magnesium.