Figure 10.

Contextual and cued fear conditioning and hot plate test. Memory consolidation was assessed with contextual and cued fear conditioning tests. Wild-type (n = 20) or relaxin-3 KO (n = 20) mice were placed in a novel environment chamber for 8 min, where they received three electrical foot shocks. Freezing time (A) and distance traveled (F) were recorded. A tone was presented for 30 s (bars) followed by a 2-s foot shock (arrowheads). Twenty-four hours later, mice were placed in the same [context; (B,D)] or modified [cue; (C,E)] chamber, and freezing time was measured as an indicator of memory consolidation. Data are presented as mean ± SEM. p-Values indicate significance of genotype effect in two-way repeated measures ANOVA. Pain sensitivity was confirmed by the hot plate test (G). Data are presented as mean ± SEM. p-Values were calculated by Student's t-test.