Figure 2.

Bacterial artificial chromosome-CGH of primary tissue culture. E. coli O157:H7 strain Sakai BAC clones with increased adherence were selected by multiple rounds of adherence to primary bovine terminal rectum cells. Total gDNA from output (adherent) and input (384 clones from the library) pools were subjected to CGH analysis. The ratio of output and input were normalized and a sliding average (window = 50 genes) applied. The black solid line indicates average ratio (output/input), the red line indicates the average p value and gray shading indicates an arbitrary two-fold cut-off for significance. Green lines and numbers above the plot indicate the location of O-islands. Solid arrowheads and designations indicate select genes implicated in adherence of E. coli.