Figure 3.

Bacterial artificial chromosome-CGH of mucus-cultured clones. E. coli O157:H7 strain Sakai BAC clones were selected cultured were selected by multiple rounds of growth in bovine terminal rectal mucus. Total gDNA from output (mucus grown) and input (384 clones from the library) pools were subjected to CGH analysis. The ratio of output and input were normalized and a sliding average (window = 50 genes) applied. The black solid line indicates average average ratio (output/input), the red line indicates the average p value and gray shading indicates an arbitrary two-fold cut-off for significance. Green lines and numbers above the plot indicate the location of O-islands. Solid arrowheads and designations indicate select genes implicated in sugar utilization of E. coli. The red dashed box indicates the region selected for further confirmation (see Figure 4).