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. Author manuscript; available in PMC: 2012 Aug 16.
Published in final edited form as: Cancer Cell. 2011 Aug 16;20(2):187–199. doi: 10.1016/j.ccr.2011.06.016

Figure 3. PRC2 silences multiple miRNAs by epigenetic mechanisms.

Figure 3

(A) Taqman miRNA qPCR analysis of indicated miRs in BT-549 and DU145 cells in which EZH2 was knocked down using siRNA or miR-101 (a microRNA which targets EZH2). Quantitative microRNA levels were normalized against U6. (B) As in (A), except DZNep at two different doses and time points was incubated with DU145 cells. (C), As in (A), except SAHA and/or 5-aza-dC was used in BT-549 and DU145 cells. (D) ChIP-qPCR analysis of H3K27me3 at indicated genes and microRNAs in BT-549 cells treated with SAHA and/or 5-aza-dC. All bar graphs are shown with ±SEM. See also Figure S3.