Figure 3.

Parvalbumin immunostaining in the tree shrew striatum. (A–C) Low magnification images of the rostral (A), mid (B), and caudal (C) striatum, showing the presence of bands of low labeling (black arrows) in these three rostrocaudal regions. (D) Detail of a low parv-ir band in the rostral caudate (black arrows). Several labeled neurons present multiple slender and long labeled processes (arrowheads), crossing in some cases from rich immunolabeled areas into the low parv-ir band. (E) Detail of parvalbumin labeling in the mid caudate showing the presence of a low immunoreactive band (black arrows) flanked by parv-ir cells distributed in clusters. Some labeled neurons are located in the low labeling band (open arrow). (F) Detail of parv-ir cells in the caudal putamen showing immunolabeled processes (arrowheads). A low immunolabeling band is located on the right and is devoid of labeled cells (black arrows). Note also in (D–F) the abundance of labeled processes in the parvalbumin rich areas, but also the presence of some processes in the areas with low parv-ir. Figures (D–F) are composite images of several Z-stack images. Coordinates indicate the orientation of the sections in the figure (D, dorsal; L, lateral). ac, anterior commissure; C, caudate; cc, corpus callosum; ic, internal capsule; GP, globus pallidus; P, putamen. Scale bars: 1 mm in (A–C); 100 μm in (D–F).