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Protein expression levels of Akt and p-Akt were analyzed by Western blotting after treatment with gemcitabine in both erlotinib-sensitive A-431 cells (parental) and erlotinib-resistant A-431 cells (two pools). p-Akt but not Akt was downregulated in both erlotinib-sensitive and erlotinib-resistant cells. β-actin was used as a loading control.
