Skip to main content
. Author manuscript; available in PMC: 2011 Aug 17.
Published in final edited form as: Nat Genet. 2010 Jul 4;42(8):668–675. doi: 10.1038/ng.613

Figure 1.

Figure 1

TOP2B is required for efficient induction of AR target gene expression following androgen stimulation. a, Inhibition of TOP2B with ET or Mer prior to DHT stimulation of LNCaP cells attenuated expression of an androgen-induced geneset (p < 10−14), defined as the 45 genes upregulated by >2-fold in DHT vs. control treated LNCaP cells. b, Geneset enrichment analysis shows highly statistically significant enrichment for down regulation of three independently derived but overlapping androgen-responsive genesets in ET/DHT vs. DHT, Mer/DHT vs. DHT, and sh-TOP2B/DHT vs DHT (see also Supplementary Fig. 2,3). Y-axis shows the degree of confidence for geneset enrichment as −log10(p-value). Significance threshold corresponding to p = 0.05 is indicated. c–d, Real-time RT-PCR analysis of relative expression of selected genes with respect to GAPDH expression confirms down-regulation of representative androgen-responsive genes in ET/DHT, Mer/DHT, and sh-TOP2B/DHT. Data are shown as mean ± SE of two to three replicates.