Table 2.

The genome constellations (internal gene segments only) of 19 LP AIV recovered from ducks sampled at the same location in Maryland, USA in 2006 without evidence of mixed infection. The different colors reflect segments whose sequences fall into different major clades as defined by strong bootstrap support (>80%) in each internal gene segment tree (see legend). A/environment/MD/1158/2006/H5N1* and A/envroinment/MD/261/2006 (H7N3) were used as the reference sequence to calculate nucleotide percent identity to identify thirteen unique clades. Shared segments are shaded in the same color (see Tables 2–4 legend for explanation of color codes).

ISOLATE	PB2	PB1	PA	NP	MP	NS	Date of Isolation
environment/MD/1158/2006 (H5N1)*							8/2/06
environment/MD/261/2006 (H7N3)*							9/8/06
environment/MD/1101/2006 (H4N6)							9/8/06
environment/MD/1655/2006 (H4N6)							9/8/06

^ξIndicates that eleven of the environmental H5N1 and four of the H7N3 collected from a single locale in the Eastern Shore of Maryland were virtually identical to the reference sequences, and were not included in the table for the sake of redundancy.

Tables 2–4 Legend. Color-coded reference sequences representing thirteen unique clades were used to trace reassortment patterns across all eight segments. Gene segments outside of these clades were designated other (orange). *A/environment/MD/1158/2006 (H5N1) and A/environment/MD/261/2006 (H7N3) were used as the reference sequences in the analyses.

Legend

A/duck/MN/1525/1981 (H5N1)

A/ruddy turnstone/NJ/65/1985 (H7N3)

A/widgeon/OH/379/1988 (H5N2)

A/wood duck/OH/623/2004 (H5N1)

A/shorebird/DE/101/2004 (H5N7)

A/blue wing teal/OH/658/2004 (H7N3)

A/environment/MD/261/2006 (H7N3)*

A/shorebird/DE/22/2006 (H7N3)

A/environment/MD/2006 (H3N6)

A/mallard/MD/182/2006 (H5N2)

A/environment/MD/1158/2006 (H5N1)*

A/mallard/MD/1235/2006 (H3N6)

A/green wing teal/CA/609/2007 (H5N2)

Other